Table 2 Primer sequences and PCR protocol
SNP | primer | Sequence | PCR Program |
|---|---|---|---|
STAT6 rs324011 | STAT6-Fwd | 5’-CTC TTC CCA CCC CTG TGT CTA TC-3’ | 1 cycle 95 °C 3 min; 35 cycles 95 °C 30 s, 67 °C 30 s, 72 °C 1 min; 1 cycle 72 °C 5 min |
STAT6-Rev | 5’-TCC CAT AGA TAG CCC TCC TAG GTA C-3’ |